Abstract
A 13.1-kilodalton protein, cysteine-rich neurotrophic factor (CRNF), was purified from the mollusk Lymnaea stagnalis by use of a binding assay on the p75 neurotrophin receptor. CRNF bound to p75 with nanomolar affinity but was not similar in sequence to neurotrophins or any other known gene product. CRNF messenger RNA expression was highest in adult foot subepithelial cells; in the central nervous system, expression was regulated by lesion. The factor evoked neurite outgrowth and modulated calcium currents in pedal motor neurons. Thus, CRNF may be involved in target-derived trophic support for motor neurons and could represent the prototype of another family of p75 ligands.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Binding, Competitive
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Calcium / metabolism
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Hemolymph / chemistry
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Humans
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Lymnaea / chemistry*
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Molecular Sequence Data
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Motor Neurons / ultrastructure
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Nerve Growth Factors / chemistry
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Nerve Growth Factors / genetics
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Nerve Growth Factors / isolation & purification
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Nerve Growth Factors / metabolism
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Nerve Growth Factors / physiology*
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Neurites / physiology
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RNA, Messenger / genetics
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RNA, Messenger / metabolism
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Receptor, Nerve Growth Factor
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Receptors, Nerve Growth Factor / metabolism*
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
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Recombinant Proteins / metabolism
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Tumor Cells, Cultured
Substances
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Nerve Growth Factors
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RNA, Messenger
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Receptor, Nerve Growth Factor
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Receptors, Nerve Growth Factor
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Recombinant Proteins
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cysteine-rich neurotrophic factor, Lymnaea stagnalis
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Calcium
Associated data
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GENBANK/U72990
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GENBANK/U73114