Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
Comparative Study
, 87 (4), 629-38

Bcl-2 Targets the Protein Kinase Raf-1 to Mitochondria

Affiliations
Comparative Study

Bcl-2 Targets the Protein Kinase Raf-1 to Mitochondria

H G Wang et al. Cell.

Abstract

A green fluorescent protein (GFP)-Raf-1 fusion protein was used to show that Bcl-2 can target this kinase to mitochondria. Active Raf-1 fused with targeting sequences from an outer mitochondrial membrane protein protected cells from apoptosis and resulted in phosphorylation of BAD, a proapoptotic Bcl-2 homolog. Plasma membrane-targeted Raf-1 did not protect from apoptosis and resulted in phosphorylation of ERK-1 and ERK-2. Untargeted active Raf-1 improved Bcl-2-mediated resistance to apoptosis, whereas a kinase-inactive Raf-1 mutant abrogated apoptosis suppression by Bcl-2. Bcl-2 can therefore target Raf-1 to mitochondrial membranes, allowing this kinase to phosphorylate BAD or possibly other protein substrates involved in apoptosis regulation.

Comment in

Similar articles

See all similar articles

Cited by 140 articles

See all "Cited by" articles

Publication types

MeSH terms

Substances

Associated data

LinkOut - more resources

Feedback