1,25-Dihydroxyvitamin D3, an inducer of glial cell line-derived neurotrophic factor

Neuroreport. 1996 Sep 2;7(13):2171-5. doi: 10.1097/00001756-199609020-00023.


Glial cell line-derived neurotrophic factor (GDNF) has significant therapeutic potentials, in particular for neurodegenerative disorders. To determine factors that would enhance GDNF expression, we analysed the effect of 1,25-(OH)2 D3 in C6 glioma cells. Treatment of C6 cells with 10(-7) M, 1,25-(OH)2 D3 for 48 h elicited an 18.5-fold increase in the level of GDNF mRNA. In addition, our results indicate that 1,25-(OH)2 D3 is effective at concentrations as low as 10(-10) M and that retinoic acid has additive effects. These data indicate that 1,25-(OH)2 D3 is a potent inducer of GDNF expression and suggest that 1,25-(OH)2 D3 may contribute to the regulation of GDNF in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcitriol / pharmacology*
  • Cell Line
  • Cell Nucleus / metabolism
  • Glial Cell Line-Derived Neurotrophic Factor
  • Glioma
  • Kinetics
  • Nerve Growth Factors / biosynthesis*
  • Nerve Tissue Proteins / biosynthesis*
  • RNA, Messenger / biosynthesis
  • Time Factors
  • Transcription, Genetic / drug effects*
  • Tretinoin / pharmacology


  • Glial Cell Line-Derived Neurotrophic Factor
  • Nerve Growth Factors
  • Nerve Tissue Proteins
  • RNA, Messenger
  • Tretinoin
  • Calcitriol