Novel domains in NADPH oxidase subunits, sorting nexins, and PtdIns 3-kinases: binding partners of SH3 domains?

Protein Sci. 1996 Nov;5(11):2353-7. doi: 10.1002/pro.5560051122.


Two SH3 domain-containing cytosolic components of the NADPH oxidase, p47phox and p40phox, are shown by analyses of their sequences to contain single copies of a novel class of domain, the PX (phox) domain. Homologous domains are demonstrated to be present in the Cpk class of phosphatidylinositol 3-kinase, S. cerevisiae Bem1p, and S. pombe Scd2, and a large family of human sorting nexin 1 (SNX1) homologues. The majority of these domains contains a polyproline motif, typical of SH3 domain-binding proteins. Two further findings are reported. A third NADPH oxidase subunit, p67phox, is shown to contain four tetratricopeptide repeats (TPRs) within its N-terminal RaclGTP-binding region, and a 28 residue motif in p40phox is demonstrated to be present in protein kinase C isoforms iota/lambda and zeta, and in three ZZ domain-containing proteins.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Carrier Proteins / chemistry
  • Carrier Proteins / metabolism*
  • Humans
  • Molecular Sequence Data
  • NADPH Oxidases / chemistry*
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor) / chemistry
  • Phosphotransferases (Alcohol Group Acceptor) / metabolism*
  • Sequence Homology, Amino Acid
  • Vesicular Transport Proteins*
  • src Homology Domains*


  • Carrier Proteins
  • Vesicular Transport Proteins
  • NADPH Oxidases
  • Phosphatidylinositol 3-Kinases
  • Phosphotransferases (Alcohol Group Acceptor)

Associated data

  • GENBANK/D11379
  • GENBANK/D24276
  • GENBANK/D28076
  • GENBANK/L11455
  • GENBANK/M25665
  • GENBANK/N48381
  • GENBANK/R21308
  • GENBANK/R56645
  • GENBANK/U00059
  • GENBANK/U12539
  • GENBANK/U33007
  • GENBANK/U38545
  • GENBANK/U51033
  • GENBANK/U52192
  • GENBANK/U52193
  • GENBANK/U53225
  • GENBANK/U55854
  • GENBANK/X66371
  • GENBANK/X77094
  • GENBANK/Z28256
  • GENBANK/Z28303
  • GENBANK/Z36069
  • GENBANK/Z44623
  • GENBANK/Z48229
  • GENBANK/Z48613
  • GENBANK/Z50142
  • GENBANK/Z69660
  • GENBANK/Z74161
  • GENBANK/Z74977
  • GENBANK/Z75265