flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis

M H Rashid; J Sekiguchi

doi:10.1128/jb.178.22.6640-6643.1996

flaD (sinR) mutations affect SigD-dependent functions at multiple points in Bacillus subtilis

J Bacteriol. 1996 Nov;178(22):6640-3. doi: 10.1128/jb.178.22.6640-6643.1996.

Authors

M H Rashid¹, J Sekiguchi

Affiliation

¹ Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University, Nagano, Japan.

Abstract

A flaD (sinR) null mutation depressed sigD-lacZ expression only two- to fourfold, whereas a flaD1 point mutation depressed it almost completely. Introduction of pHYSigD, a sigmaD-overproducing plasmid, corrected the filamentous phenotype common to both sinR mutants; autolysin synthesis was restored partially and completely in the flaD1 and flaD (sinR) null strains, respectively. Flagellin synthesis and motility were not restored at all in either strain.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacillus subtilis / cytology
Bacillus subtilis / genetics*
Bacterial Proteins / genetics*
DNA-Directed RNA Polymerases / metabolism*
Flagellin / biosynthesis
Gene Expression Regulation, Bacterial*
Mutation*
N-Acetylmuramoyl-L-alanine Amidase / biosynthesis
Sigma Factor / metabolism*

Substances

Bacterial Proteins
Sigma Factor
Flagellin
FlaD protein, Bacteria
RNA polymerase sigma 70
DNA-Directed RNA Polymerases
N-Acetylmuramoyl-L-alanine Amidase