Novel phosphotransferase genes revealed by bacterial genome sequencing: a gene cluster encoding a putative N-acetylgalactosamine metabolic pathway in Escherichia coli

Microbiology. 1996 Feb;142 ( Pt 2):231-50. doi: 10.1099/13500872-142-2-231.

Abstract

We have analysed a gene cluster in the 67 center dot 4-76 center dot 0 min region of the Escherichia coli chromosome, revealed by recent systematic genome sequencing. The genes within this cluster include: (1) five genes encoding homologues of the E. coli mannose permease of the phosphotransferase system (IIB, IIB', IIC, IIC' and IID); (2) genes encoding a putative N-acetylgalactosamine 6-phosphate metabolic pathway including (a) a deacetylase, (b) an isomerizing deaminase, (c) a putative carbohydrate kinase, and (d) an aldolase; and (3) a transcriptional regulatory protein homologous to members of the DeoR family. Evidence is presented suggesting that the aldolase-encoding gene within this cluster is the previously designated kba gene that encodes tagatose-1,6-bisphosphate aldolase. These proteins and a novel IIAMan-like protein encoded in the 2 center dot 4-4 center dot 1 min region are characterized with respect to their sequence similarities and phylogenetic relationships with other homologous proteins. A pathway for the metabolism of N-acetylgalactosamine biochemically similar to that for the metabolism of N-acetylglucosamine is proposed.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylgalactosamine / metabolism*
  • Amino Acid Sequence
  • Bacterial Proteins / genetics
  • Base Sequence
  • Chromosome Mapping
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics*
  • Escherichia coli / metabolism*
  • Genes, Bacterial*
  • Molecular Sequence Data
  • Multigene Family*
  • Open Reading Frames
  • Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
  • Phosphoenolpyruvate Sugar Phosphotransferase System / metabolism*
  • Phylogeny
  • Sequence Homology, Amino Acid

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • Acetylgalactosamine