The dnrM gene in Streptomyces peucetius contains a naturally occurring frameshift mutation that is suppressed by another locus outside of the daunorubicin-production gene cluster

Microbiology. 1996 Feb;142 ( Pt 2):269-75. doi: 10.1099/13500872-142-2-269.


A 2 center dot 7 kb BamHI fragment of the daunorubicin biosynthetic cluster in Streptomyces peucetius ATCC 29050 was shown to contain two ORFs, dnrL and dnrM, whose deduced products exhibit a high sequence similarity to a number of glucose-1-phosphate thymidylyl transferases and TDP-D-glucose dehydratases, respectively. Although these genes were believed to be necessary for the synthesis of the deoxyaminosugar, daunosamine, a constituent of daunorubicin, the dnrM gene contains a frameshift in the DNA sequence that causes the premature termination of translation. A gene encoding another TDP-glucose 4,6-dehydratase, previously isolated from S. peucetius, was identified by PCR amplification of genomic DNA. The presence of this gene explains why a dnrM::aphll mutation did not block daunorubicin production.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • DNA Primers / genetics
  • DNA, Bacterial / genetics
  • Daunorubicin / biosynthesis*
  • Escherichia coli / genetics
  • Frameshift Mutation*
  • Genes, Bacterial*
  • Genes, Suppressor
  • Hydro-Lyases / genetics
  • Molecular Sequence Data
  • Multigene Family*
  • Nucleotidyltransferases / genetics
  • Polymerase Chain Reaction
  • Streptomyces / genetics*
  • Streptomyces / metabolism


  • DNA Primers
  • DNA, Bacterial
  • Nucleotidyltransferases
  • glucose-1-phosphate thymidylyltransferase
  • Hydro-Lyases
  • dTDPglucose 4,6-dehydratase
  • Daunorubicin

Associated data

  • GENBANK/L47163