Taxol from Pestalotiopsis microspora, an endophytic fungus of Taxus wallachiana

Microbiology. 1996 Feb;142 ( Pt 2):435-40. doi: 10.1099/13500872-142-2-435.

Abstract

Pestalotiopsis microspora was isolated from the inner bark of a small limb of Himalayan yew, Taxus wallachiana, and was shown to produce taxol in mycelial culture. Taxol was identified by spectroscopic and chromatographic comparisons with authentic taxol. Optimal taxol production occurred after 2-3 weeks in still culture at 23 degrees C. [14C]Acetate and [14C]phenylalanine served as precursors for fungal [14C]taxol. These observations on P. microspora are discussed in relation to the biological importance of taxol production by fungi in general.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetic Acid / metabolism
  • Antineoplastic Agents, Phytogenic / biosynthesis*
  • Antineoplastic Agents, Phytogenic / chemistry
  • Antineoplastic Agents, Phytogenic / pharmacology
  • Biotechnology
  • Drug Screening Assays, Antitumor
  • Ecosystem
  • Fermentation
  • Humans
  • Magnetic Resonance Spectroscopy
  • Mass Spectrometry
  • Microscopy, Electron, Scanning
  • Mitosporic Fungi / isolation & purification*
  • Mitosporic Fungi / metabolism*
  • Mitosporic Fungi / ultrastructure
  • Paclitaxel / biosynthesis*
  • Paclitaxel / chemistry
  • Paclitaxel / pharmacology
  • Phenylalanine / metabolism
  • Spores, Fungal / ultrastructure
  • Trees / microbiology*
  • Tumor Cells, Cultured

Substances

  • Antineoplastic Agents, Phytogenic
  • Phenylalanine
  • Paclitaxel
  • Acetic Acid