Epitopes of Bordetella pertussis lipopolysaccharides as potential markers for typing of isolates with monoclonal antibodies

Microbiology (Reading). 1996 Apr;142 ( Pt 4):971-978. doi: 10.1099/00221287-142-4-971.


Three hybridomas (P1P3, D7 and 60.5) producing monoclonal antibodies (mAbs) against Bordetella pertussis lipopolysaccharide (LPS) were established. All reacted with the LPS from a typical, vaccine strain of B. pertussis (1414), but not with that of a variant strain (A100). Two of these mAbs (P1P3 and 60.5) cross-reacted with a B. bronchiseptica LPS; only one (P1P3) reacted with a B. parapertussis LPS. ELISA reactivities with intact LPSs, and defined partial structures covalently linked to bovine serum albumin, were compared. mAb 60.5 bound to the terminal region of a distal trisaccharide consisting of N-acetylated amino sugars. D7 reacted with a substructure which can be modified in the B. parapertussis and B. bronchiseptica LPSs by addition of a polymeric O-chain. P1P3 bound to a nonacetylated glucosamine substituted with L-glycero-D-manno-heptose, present in the 'core' of the B. pertussis LPS. These mAbs may be useful for rapid typing of Bordetella in clinical isolates.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / biosynthesis
  • Antibodies, Monoclonal*
  • Antigens, Bacterial* / chemistry
  • Bacterial Typing Techniques*
  • Biomarkers
  • Bordetella / immunology
  • Bordetella pertussis / classification*
  • Bordetella pertussis / immunology*
  • Carbohydrate Sequence
  • Cattle
  • Cross Reactions
  • Epitopes / chemistry
  • Hybridomas / immunology
  • Lipopolysaccharides / chemistry
  • Lipopolysaccharides / immunology*
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Molecular Structure
  • Serum Albumin, Bovine / immunology


  • Antibodies, Bacterial
  • Antibodies, Monoclonal
  • Antigens, Bacterial
  • Biomarkers
  • Epitopes
  • Lipopolysaccharides
  • Serum Albumin, Bovine