The incorporation of Ga-67 into the ferritin fraction of rabbit hepatocytes in vivo

J Nucl Med. 1977 Sep;18(9):937-9.

Abstract

Twenty-four hours after the administration of Ga-67 citrate and Fe-59 citrate, rabbits were killed and their livers removed and homogenized. Labile proteins in the filtered liver homogenates were denatured; ferritin was then crystallized from the supernatants by cadmium sulfate. Sephadex G-200 gel filtration of the ferritin fractions was done to determine the distribution of molecular weights in the substances associated with Ga-67 and Fe-59. It was found that Ga-67 was incorporated into the crystallizable ferritin fraction of rabbit hepatocytes with approximately one-sixth the uptake of simultaneously administered Fe-59. Gel-filtration chromatography confirmed that both the Ga-67 and the Fe-59 of the crystallizable ferritin fraction were associated with substances of the appropriate molecular weight for ferritin.

MeSH terms

  • Animals
  • Ferritins / analysis*
  • Gallium Radioisotopes / analysis*
  • Iron Radioisotopes / analysis
  • Liver / metabolism*
  • Protein Binding
  • Rabbits

Substances

  • Gallium Radioisotopes
  • Iron Radioisotopes
  • Ferritins