Cloning, RNA expression, and chromosomal location of a mouse histamine H2 receptor gene

Genomics. 1996 Nov 1;37(3):390-4. doi: 10.1006/geno.1996.0575.


A mouse histamine H2 receptor (H2R) gene was isolated from a mouse ES cell genomic DNA library. The gene encoded a 358-amino-acid protein and displayed a 95% homology with the rat histamine H2 receptor at the amino acid level. It had features characteristic of other G-protein-coupled receptors. Reverse transcriptase-polymerase chain reaction analysis of total RNA prepared from mouse tissues showed that the gene was highly expressed in stomach and moderately in brain and heart. A weak expression was also detected in liver. An interspecific backcross analysis revealed that the mouse H2R gene, designated Hrh2, was located in the central region of chromosome 13.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Brain / metabolism
  • Chromosome Mapping
  • Cloning, Molecular
  • Crosses, Genetic
  • Dogs
  • Female
  • Gastric Mucosa / metabolism
  • Gene Expression
  • Gene Library
  • Genes*
  • Humans
  • Male
  • Mice / genetics*
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Muridae / genetics
  • Myocardium / metabolism
  • Organ Specificity
  • Polymerase Chain Reaction
  • RNA, Messenger / genetics
  • Rats
  • Receptors, Histamine H2 / biosynthesis
  • Receptors, Histamine H2 / genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Species Specificity


  • RNA, Messenger
  • Receptors, Histamine H2

Associated data

  • GENBANK/D50096