Preparations of duck hepatitis B virions contain multiple DNA polymerase activities

Virology. 1996 Dec 1;226(1):132-4. doi: 10.1006/viro.1996.0636.


The hepadnaviral DNA genome is synthesized by a viral-encoded reverse transcriptase, but the nature of this protein(s) in vivo remains obscure. We have previously described studies in which activity gel assays identified multiple DNA polymerase (DNAp) activities associated with highly purified duck hepatitis B virus (DHBV) core particles. We now report that virions isolated from viremic sera are associated with DNA-dependent DNAp activities which are nearly identical to major DNAp activities detected with highly purified DHBV core particles. These results suggest that the virion-associated polymerases are the same as those which are detected with core particles and are likely to represent DHBV pol gene products involved in replication of the genome.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • DNA-Directed DNA Polymerase / chemistry
  • DNA-Directed DNA Polymerase / metabolism*
  • Ducks / virology
  • Hepadnaviridae Infections / blood
  • Hepadnaviridae Infections / virology
  • Hepatitis B Virus, Duck / enzymology*
  • Hepatitis B Virus, Duck / isolation & purification
  • Viral Proteins / chemistry
  • Viral Proteins / metabolism*
  • Virion / metabolism


  • Viral Proteins
  • DNA-Directed DNA Polymerase