The aim of this study was to ascertain the prevalence of Lyme disease as a cause of annular erythematous eruptions in patients attending the National Skin Centre. Serum samples of 72 patients presenting with annular erythema were tested for syphilis, and antibodies against Berrelia burgdorferi by haemagglutination, indirect immunofluorescence and enzyme-linked immunosorbent tests. All the serum tested were negative for syphilis serology and antibody against B. burgdorferi. It would appear that the Lyme disease is not a cause of annular erythema in Singapore.