Chromatographic method for the determination of non-transferrin-bound iron suitable for use on the plasma and bronchoalveolar lavage fluid of preterm babies

Clin Sci (Lond). 1996 Nov;91(5):633-8. doi: 10.1042/cs0910633.


1. Non-protein-bound iron has been implicated in the aetiology of chronic lung disease of prematurity. 2. The modification of a method for the measurement of non-transferrin-bound iron in small volumes of plasma and bronchoalveolar lavage fluid from preterm babies is described. 3. The assay runs with a good degree of precision and a lower limit of detection of 0.02 mumol/l. 4. Non-transferrin-bound iron was detected in 50% of plasma samples and 11% of bronchoalveolar lavage fluid samples collected over the first week of life from babies born prematurely.

Publication types

  • Clinical Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bronchoalveolar Lavage Fluid / chemistry*
  • Chromatography, High Pressure Liquid / methods
  • Humans
  • Infant, Newborn
  • Infant, Premature / blood
  • Infant, Premature / metabolism*
  • Iron / analysis*
  • Iron / blood
  • Iron / metabolism
  • Transferrin / metabolism*


  • Transferrin
  • Iron