Abstract
Stress responses in plants involve changes in the transcription of specific genes. The constitutively active mutants of two related Ca2+-dependent protein kinases (CDPK1 and CDPK1a) activate a stress-inducible promoter, bypassing stress signals. Six other plant protein kinases, including two distinct CDPKs, fail to mimic this stress signaling. The activation is abolished by a CDPK1 mutation in the kinase domain and diminished by a constitutively active protein phosphatase 2C that is capable of blocking responses to the stress hormone abscisic acid. A variety of functions are mediated by different CDPKs. CDPK1 and CDPK1a may be positive regulators controlling stress signal transduction in plants.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Abscisic Acid / pharmacology
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Amino Acid Sequence
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Arabidopsis / enzymology
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Arabidopsis / genetics
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Arabidopsis Proteins*
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Calcium / metabolism
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Calcium-Binding Proteins / genetics
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Calcium-Binding Proteins / metabolism*
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DNA, Complementary / genetics
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Gene Expression Regulation, Plant
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Genes, Reporter
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Green Fluorescent Proteins
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Luminescent Proteins / genetics
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Molecular Sequence Data
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Mutagenesis, Site-Directed
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Phosphoprotein Phosphatases / metabolism
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Plant Leaves / metabolism
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Plant Proteins / genetics*
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Promoter Regions, Genetic*
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Protein Kinases / genetics
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Protein Kinases / metabolism*
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Protein Phosphatase 2
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Protein Phosphatase 2C
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Protoplasts / metabolism
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Saccharomyces cerevisiae Proteins*
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Signal Transduction*
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Transfection
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Zea mays / genetics
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Zea mays / metabolism*
Substances
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ATCDPK1 protein, Arabidopsis
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Arabidopsis Proteins
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Calcium-Binding Proteins
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DNA, Complementary
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HVA1 protein, Hordeum vulgare
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Luminescent Proteins
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Plant Proteins
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Saccharomyces cerevisiae Proteins
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Green Fluorescent Proteins
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Abscisic Acid
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Protein Kinases
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PTC1 protein, S cerevisiae
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Phosphoprotein Phosphatases
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Protein Phosphatase 2
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Protein Phosphatase 2C
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Calcium