Identification of a RING protein that can interact in vivo with the BRCA1 gene product

Nat Genet. 1996 Dec;14(4):430-40. doi: 10.1038/ng1296-430.


The hereditary breast and ovarian cancer gene, BRCA1, encodes a large polypeptide that contains the cysteine-rich RING motif, a zinc-binding domain found in a variety of regulatory proteins. Here we describe a novel protein that interacts in vivo with the N-terminal region of BRCA1. This BRCA1-associated RING domain (BARD1) protein contains an N-terminal RING motif, three tandem ankyrin repeats, and a C-terminal sequence with significant homology to the phylogenetically conserved BRCT domains that lie near the C terminus of BRCA1. The BARD1/BRCA1 interaction is disrupted by BRCA1 missense mutations that segregate with breast cancer susceptibility, indicating that BARD1 may be involved in mediating tumour suppression by BRCA1.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Ankyrins / metabolism
  • BRCA1 Protein / genetics
  • BRCA1 Protein / metabolism*
  • Binding Sites
  • Breast Neoplasms / metabolism
  • Carrier Proteins / genetics
  • Carrier Proteins / metabolism*
  • Cell Line
  • Escherichia coli / genetics
  • Female
  • HeLa Cells
  • Humans
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Ovarian Neoplasms / metabolism
  • Protein Binding
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / metabolism
  • Saccharomyces cerevisiae / genetics
  • Sequence Homology, Amino Acid
  • Tumor Cells, Cultured
  • Tumor Suppressor Proteins*
  • Ubiquitin-Protein Ligases*


  • Ankyrins
  • BRCA1 Protein
  • Carrier Proteins
  • Recombinant Fusion Proteins
  • Tumor Suppressor Proteins
  • BARD1 protein, human
  • Ubiquitin-Protein Ligases

Associated data

  • GENBANK/L25444
  • GENBANK/L29277
  • GENBANK/U20657
  • GENBANK/U31659
  • GENBANK/U57317
  • GENBANK/U76638
  • GENBANK/X53416