Identification of BTG2, an antiproliferative p53-dependent component of the DNA damage cellular response pathway

Nat Genet. 1996 Dec;14(4):482-6. doi: 10.1038/ng1296-482.


Cell cycle regulation is critical for maintenance of genome integrity. A prominent factor that guarantees genomic stability of cells is p53 (ref. 1). The P53 gene encodes a transcription factor that has a role as a tumour suppressor. Identification of p53-target genes should provide greater insight into the molecular mechanisms that mediate the tumour suppressor activities of p53. The rodent Pc3/Tis21 gene was initially described as an immediate early gene induced by tumour promoters and growth factors in PC12 and Swiss 3T3 cells. It is expressed in a variety of cell and tissue types and encodes a remarkably labile protein. Pc3/Tis21 has a strong sequence similarity to the human antiproliferative BTG1 gene cloned from a chromosomal translocation of a B-cell chronic lymphocytic leukaemia. This similarity led us to speculate that BTG1 and the putative human homologue of Pc3/Tis21 (named BTG2) were members of a new family of genes involved in growth control and/or differentiation. This hypothesis was recently strengthened by the identification of a new antiproliferative protein, named TOB, which shares sequence similarity with BTG1 and PC3/TIS21 (ref. 7). Here, we cloned and localized the human BTG2 gene. We show that BTG2 expression is induced through a p53-dependent mechanism and that BTG2 function may be relevant to cell cycle control and cellular response to DNA damage.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 3T3 Cells
  • Amino Acid Sequence
  • Animals
  • Cell Cycle / genetics
  • Cell Cycle / physiology
  • Cell Division / physiology*
  • Cell Line
  • Chromosome Mapping
  • Chromosomes, Human, Pair 1
  • Cloning, Molecular
  • DNA Damage*
  • Gene Expression Regulation
  • Genes, Tumor Suppressor
  • Humans
  • Hybrid Cells
  • Immediate-Early Proteins*
  • Mice
  • Molecular Sequence Data
  • Neoplasm Proteins / genetics
  • Proteins / genetics*
  • Proteins / physiology
  • Sequence Homology, Amino Acid
  • Tumor Suppressor Protein p53 / physiology*
  • Tumor Suppressor Proteins


  • Btg1 protein, mouse
  • Btg2 protein, mouse
  • Immediate-Early Proteins
  • Neoplasm Proteins
  • Proteins
  • Tumor Suppressor Protein p53
  • Tumor Suppressor Proteins
  • BTG2 protein, human
  • BTG1 protein, human