Renal organic anion secretion: evidence for dopaminergic and adrenergic regulation

Am J Physiol. 1996 Nov;271(5 Pt 2):R1372-9. doi: 10.1152/ajpregu.1996.271.5.R1372.


To examine possible regulatory control of renal proximal tubule organic anion secretion, winter flounder (Pleuronectes americanus) proximal tubule primary cultures were mounted in Ussing chambers. Unidirectional fluxes of [2,4-(14)C]dichlorophenoxyacetic acid were determined under short-circuited conditions. Phorbol 12-myristate 13-acetate (1 microM) caused a significant (P < 0.01) inhibition of net 2,4-dichlorophenoxyacetic acid secretion. Preincubation with staurosporine (1 microM) blocked the phorbol 12-myristate 13-acetate-induced decrease in secretion. Neither forskolin (10 microM) nor W-7 (20 microM) had any effect on net transport. Elevation of intracellular calcium activity with either A-23187 or thapsigargin produced a slight, transient decrease in transport. Addition of dopamine (1 microM) to the peritubular side, but not the luminal side, caused a significant (P < 0.01) decrease in net secretion. Both the alpha-adrenergic agonist oxymetazoline (10 microM) and depletion of intracellular Na+ transiently, but significantly (P < 0.05), increased net transport. The data indicate that renal organic anion excretion may be regulated through dopaminergic inhibition and alpha-adrenergic stimulation of net transepithelial secretion.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 2,4-Dichlorophenoxyacetic Acid / antagonists & inhibitors
  • 2,4-Dichlorophenoxyacetic Acid / metabolism
  • Adrenergic Agents / pharmacology*
  • Adrenergic alpha-Agonists / pharmacology
  • Animals
  • Anions / metabolism*
  • Calcium / metabolism
  • Dopamine / pharmacology*
  • Enzyme Activation
  • Flounder
  • Intracellular Membranes / metabolism
  • Kidney Tubules, Proximal / drug effects
  • Kidney Tubules, Proximal / metabolism*
  • Oxymetazoline / pharmacology
  • Probenecid / pharmacology
  • Protein Kinases / metabolism


  • Adrenergic Agents
  • Adrenergic alpha-Agonists
  • Anions
  • 2,4-Dichlorophenoxyacetic Acid
  • Oxymetazoline
  • Protein Kinases
  • Probenecid
  • Calcium
  • Dopamine