Detection and immunolocalization of macrophage migration inhibitory factor in rat iris and ciliary epithelium

Immunol Lett. 1996 Oct;53(1):1-5. doi: 10.1016/0165-2478(96)02600-4.


To elucidate the role of macrophage migration inhibitory factor (MIF) in ocular inflammation, we examined the localization of MIF in the normal anterior uveal tract of rats. Immunohistochemistry using an anti-MIF antibody revealed that MIF was present in non-pigment epithelial cells of the ciliary body and the epithelial cells of the iris. Western blot analysis of these tissues showed a single band specific for MIF protein. The expression of MIF mRNA in these tissues was further confirmed by reverse transcription-polymerase chain reaction. Since MIF is known to be a potent proinflammatory cytokine, identification of the protein in iris and ciliary epithelial cells suggests the possibility that it may play an important role in ocular inflammation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Ciliary Body / immunology*
  • Epithelium
  • Immunoenzyme Techniques
  • Iris / immunology*
  • Macrophage Migration-Inhibitory Factors / genetics
  • Macrophage Migration-Inhibitory Factors / immunology*
  • Polymerase Chain Reaction
  • Rabbits
  • Rats
  • Rats, Wistar
  • Transcription, Genetic


  • Macrophage Migration-Inhibitory Factors