A novel carbon fiber bundle microelectrode and modified brain slice chamber for recording long-term multiunit activity from brain slices

J Neurosci Methods. 1996 Nov;69(2):163-9. doi: 10.1016/S0165-0270(96)00039-8.

Abstract

The fabrication and characteristics of a novel multiunit recording electrode and modified brain slice chamber suitable for long-term recording from brain slices are described. The electrode consisted of an electrolyte-filled glass micropipette with a 20-50 microns thick wax-coated bundle of 5-micron diameter carbon fibers extending 2.5 cm from the tapered end and an AgCl-coated silver wire inserted into the open end and connected to a preamplifier. Both ends of the electrode were sealed with wax to prevent evaporation of the electrolyte. The brain slice was maintained over this extended period in an interface-type brain slice chamber modified to completely surround the slice with medium. Using this electrode, regular 24-h oscillations of spontaneous multiunit activity were recorded for 3 days from a single location in a 500 microns thick rat suprachiasmatic nucleus brain slice. Preliminary data suggest that this novel carbon fiber bundle electrode will be a favorable alternative to traditional metal electrodes for long-term recording of multiunit activity from brain slices.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Carbon*
  • Electrophysiology / instrumentation*
  • Electrophysiology / methods
  • Female
  • Male
  • Microelectrodes*
  • Organ Culture Techniques
  • Rats
  • Rats, Inbred Strains
  • Suprachiasmatic Nucleus / physiology*

Substances

  • Carbon