Limited T helper cell activity in C57BL/10 (B10) mice with inherited low IgG responsiveness

Scand J Immunol. 1996 Nov;44(5):453-60. doi: 10.1046/j.1365-3083.1996.d01-337.x.


Due to limitations in antigen processing, mice of the C57BL/10ScSn (B10) strain exhibit a low IgG production against a variety of T-dependent antigens. To characterize the T-cell functions, the authors studied antigen-specific T-cell proliferation and cytokine production in vitro. The response of B10 mice was compared with that of the high IgG producing strain A/J. A highly restricted proliferative response and almost no interleukin-2 (IL-2) and interleukin-3 (IL-3) production was detected in lymph node (LN) cells of B10 mice primed in vivo by protein antigens and subjected to a specific restimulation in vitro, whilst A/J cells responded by significant proliferation and cytokine production. The antigen-specific T-cell response of B10 mice could not be increased by lipopolysaccharide treatment in vivo or by in vitro cultivation with IL-2. However, the T cells of B10 mice produced high levels of IL-2 and IL-4 when stimulated by phorbol myristate acetate (PMA) and Ca2+ ionophore, proving the existence of a functionally intact signal transduction pathway downstream of protein kinase C (PKC). The results suggest that the in vivo antigen priming does not effectively activate the T cells in B10 mice. The limited activation consequently leads to the low IgG response described in B10 mice.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibody Formation / genetics*
  • Antigen Presentation / genetics*
  • Antigens / immunology
  • Calcimycin / pharmacology
  • Calcium / physiology
  • Chickens
  • Egg Proteins / immunology
  • Immunization
  • Immunoglobulin G / biosynthesis*
  • Immunoglobulin G / genetics
  • Interleukin-2 / metabolism
  • Interleukin-3 / metabolism
  • Interleukin-4 / metabolism
  • Ionophores / pharmacology
  • Lipopolysaccharides / pharmacology
  • Lymphocyte Activation
  • Lymphocyte Cooperation
  • Mice
  • Mice, Inbred A
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Mice, Mutant Strains
  • Muramidase / immunology
  • Protein Kinase C / metabolism
  • Signal Transduction / drug effects
  • T-Lymphocytes, Helper-Inducer / immunology*
  • Tetradecanoylphorbol Acetate / pharmacology


  • Antigens
  • Egg Proteins
  • Immunoglobulin G
  • Interleukin-2
  • Interleukin-3
  • Ionophores
  • Lipopolysaccharides
  • Interleukin-4
  • Calcimycin
  • Protein Kinase C
  • Muramidase
  • Tetradecanoylphorbol Acetate
  • Calcium