Expression and regulation of constitutive and acute phase serum amyloid A mRNAs in hepatic and non-hepatic cell lines

Scand J Immunol. 1996 Nov;44(5):493-500. doi: 10.1046/j.1365-3083.1996.d01-341.x.

Abstract

'Acute phase' and 'constitutive' SAA (A-SAA and C-SAA, respectively) mRNA levels were measured in hepatic and non-hepatic cell lines after treatment with monocyte conditioned medium (MoCM), with or without dexamethasone (Dex). A-SAA mRNAs were detected in MoCM-treated hepatoma cell lines (PLC/PRF/5, HuH7, HepG2, and Hep3B), a fibroblast cell line (MRC5), six epithelial cell lines (RT4/ 31, SW13, Hela Ohio, HCT-8, CaCo2, and KB), and an endothelial cell line ECV304. In KB cells, Dex alone caused a dramatic increase in A-SAA mRNA levels. C-SAA was detected in all hepatic and non-hepatic cell lines. Two differentially regulated size classes of C-SAA mRNA were detected in the hepatoma cell lines. A-SAA mRNA levels were measured in ECV304 cells treated with IL-1 beta, IL-6, TNF alpha and Dex, in various combinations, and revealed different profiles to those seen for hepatic cells. The extent of polyadenylation of A-SAA mRNA in ECV304 and KB cells differed whereas the polyadenylation of C-SAA mRNA remained constant. These data suggest that the parameters that determine the steady state mRNA levels and post-transcriptional regulation of A-SAA and C-SAA mRNAs are different and are cell type specific.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute-Phase Reaction / genetics*
  • Adrenal Cortex Neoplasms / metabolism
  • Adrenal Cortex Neoplasms / pathology
  • Carcinoma, Hepatocellular / metabolism
  • Carcinoma, Hepatocellular / pathology
  • Cells, Cultured
  • Culture Media, Conditioned / pharmacology
  • Dexamethasone / pharmacology
  • Endothelium, Vascular / cytology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / metabolism
  • Epithelial Cells
  • Epithelium / metabolism
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Gene Expression Regulation* / drug effects
  • Gene Expression Regulation, Neoplastic / drug effects
  • Half-Life
  • HeLa Cells / drug effects
  • HeLa Cells / metabolism
  • Humans
  • Interleukin-1 / pharmacology
  • Interleukin-6 / pharmacology
  • Intestinal Neoplasms / metabolism
  • Intestinal Neoplasms / pathology
  • KB Cells / drug effects
  • KB Cells / metabolism
  • Liver / metabolism*
  • Liver Neoplasms / metabolism
  • Liver Neoplasms / pathology
  • Lymphoma, Large B-Cell, Diffuse / metabolism
  • Lymphoma, Large B-Cell, Diffuse / pathology
  • Monocytes / metabolism
  • Mouth Neoplasms / metabolism
  • Mouth Neoplasms / pathology
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics
  • Organ Specificity
  • RNA Processing, Post-Transcriptional
  • RNA, Messenger / biosynthesis*
  • RNA, Messenger / genetics
  • RNA, Neoplasm / biosynthesis
  • RNA, Neoplasm / genetics
  • Recombinant Proteins / pharmacology
  • Serum Amyloid A Protein / biosynthesis*
  • Serum Amyloid A Protein / genetics
  • Tumor Cells, Cultured
  • Tumor Necrosis Factor-alpha / pharmacology

Substances

  • Culture Media, Conditioned
  • Interleukin-1
  • Interleukin-6
  • Neoplasm Proteins
  • RNA, Messenger
  • RNA, Neoplasm
  • Recombinant Proteins
  • Serum Amyloid A Protein
  • Tumor Necrosis Factor-alpha
  • Dexamethasone