Abstract
The cDNA for mouse NAD+ dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) was isolated from a lung cDNA library. The cDNA contains a 798 bp open reading frame that codes for a protein of 266 amino acids (M(r) 28775) which shares 87% identity with the human 15-PGDH protein. The regions that are believed to form the NAD+ binding site and the active site are conserved in the mouse and human enzymes. The authenticity of the mouse cDNA was confirmed by expression of an active 15-PGDH in Escherichia coli. Northern blot analysis demonstrated that 15-PGDH mRNA is expressed primarily in lung, intestine, stomach and liver.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Binding Sites
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Cloning, Molecular
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DNA, Complementary / genetics*
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Escherichia coli / genetics
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Gene Expression Regulation, Enzymologic*
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Humans
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Hydroxyprostaglandin Dehydrogenases / genetics*
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Hydroxyprostaglandin Dehydrogenases / metabolism
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Lung / enzymology
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Mice
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Molecular Sequence Data
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NAD / metabolism
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Organ Specificity
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RNA, Messenger / analysis
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Recombinant Fusion Proteins
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Sequence Homology, Amino Acid
Substances
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DNA, Complementary
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RNA, Messenger
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Recombinant Fusion Proteins
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NAD
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Hydroxyprostaglandin Dehydrogenases
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15-hydroxyprostaglandin dehydrogenase