Cloning and expression of the cDNA for mouse NAD(+)-dependent 15-hydroxyprostaglandin dehydrogenase

Biochim Biophys Acta. 1996 Nov 11;1309(1-2):21-4. doi: 10.1016/s0167-4781(96)00123-6.

Abstract

The cDNA for mouse NAD+ dependent 15-hydroxyprostaglandin dehydrogenase (15-PGDH) was isolated from a lung cDNA library. The cDNA contains a 798 bp open reading frame that codes for a protein of 266 amino acids (M(r) 28775) which shares 87% identity with the human 15-PGDH protein. The regions that are believed to form the NAD+ binding site and the active site are conserved in the mouse and human enzymes. The authenticity of the mouse cDNA was confirmed by expression of an active 15-PGDH in Escherichia coli. Northern blot analysis demonstrated that 15-PGDH mRNA is expressed primarily in lung, intestine, stomach and liver.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Cloning, Molecular
  • DNA, Complementary / genetics*
  • Escherichia coli / genetics
  • Gene Expression Regulation, Enzymologic*
  • Humans
  • Hydroxyprostaglandin Dehydrogenases / genetics*
  • Hydroxyprostaglandin Dehydrogenases / metabolism
  • Lung / enzymology
  • Mice
  • Molecular Sequence Data
  • NAD / metabolism
  • Organ Specificity
  • RNA, Messenger / analysis
  • Recombinant Fusion Proteins
  • Sequence Homology, Amino Acid

Substances

  • DNA, Complementary
  • RNA, Messenger
  • Recombinant Fusion Proteins
  • NAD
  • Hydroxyprostaglandin Dehydrogenases
  • 15-hydroxyprostaglandin dehydrogenase

Associated data

  • GENBANK/U44389