Gadolinium blocks mechano-electric transducer current in chick cochlear hair cells

Hear Res. 1996 Nov 1;101(1-2):75-80. doi: 10.1016/s0378-5955(96)00134-7.


We investigated the effects of gadolinium ion (Gd3+) on the mechano-electrical transduction (MET) current using a whole-cell patch electrode voltage clamp technique in dissociated cochlear hair cells of chicks. Gd3+ blocked the MET channel in a concentration- and voltage-dependent manner. At -50 mV, Gd3+ blocked the MET channel, with a Hill coefficient of 1.14 and a dissociation constant (KD) of 1.01 x 10(-5) M. Adaptation of the MET current disappeared after the introduction of Gd3+, a change that may be due to a decrease in inward going MET currents, specifically the Ca2+ component.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Calcium / metabolism*
  • Cell Separation
  • Chickens
  • Cochlea / cytology*
  • Cochlea / drug effects
  • Dose-Response Relationship, Drug
  • Electric Stimulation
  • Gadolinium / toxicity*
  • Hair Cells, Auditory / cytology
  • Hair Cells, Auditory / drug effects*
  • Patch-Clamp Techniques
  • Signal Transduction / drug effects*


  • Gadolinium
  • Calcium