The Drosophila TGF-alpha-like protein Gurken: expression and cellular localization during Drosophila oogenesis

Mech Dev. 1996 Oct;59(2):105-13. doi: 10.1016/0925-4773(96)00567-9.


The establishment of anterior-posterior and dorsal-ventral polarity of the Drosophila egg and embryo depends on the function of the genes gurken, cornichon and Egfr (Drosophila epidermal growth factor receptor homolog). These genes encode components of a signal transduction pathway that transmits information between the germline cells and the somatic follicle cells of the ovary. gurken encodes a transforming growth factor-alpha-like protein and is a putative germline ligand of the Egfr present on the follicle cells. In mid-oogenesis the gurken transcript becomes spatially localized to the future dorsal-anterior cortex of the oocyte. To analyze the distribution pattern of Gurken protein we prepared antibodies against Gurken. We describe here the distribution pattern of the Gurken protein in wild-type ovaries and in ovaries from a number of dorsal-ventral patterning mutants. By immunoblotting we detect one major form of the Gurken protein, which likely corresponds to the unprocessed protein.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antibodies
  • Drosophila
  • Drosophila Proteins*
  • Female
  • Genes, erbB-1 / genetics
  • Insect Proteins / metabolism*
  • Molecular Weight
  • Mutagenesis
  • Oogenesis*
  • Recombinant Fusion Proteins / metabolism
  • Transforming Growth Factor alpha / chemistry*
  • Transforming Growth Factors / metabolism*


  • Antibodies
  • Drosophila Proteins
  • Insect Proteins
  • Recombinant Fusion Proteins
  • Transforming Growth Factor alpha
  • grk protein, Drosophila
  • Transforming Growth Factors