Two novel RXR alpha isoforms from mouse testis

Biochem Biophys Res Commun. 1996 Dec 4;229(1):211-8. doi: 10.1006/bbrc.1996.1782.


We report the isolation from mouse testis cDNA of two novel RXR alpha isoforms, mRXR alpha 2 and mRXR alpha 3, with distinct sequences upstream of exon 2. These two isoforms encode a similar protein (mRXR alpha 2/3) which lacks that 28 N-terminal amino acid residues of the major RXR alpha isoform, mRXR alpha 1. The N-terminal activation function (AF-1) of mRXR alpha 2/3 appears altered when compared to that of mRXR alpha 1. mRXR alpha 2 and mRXR alpha 3 are specifically expressed in the testis, and their expression is strongly upregulated in this tissue at puberty. These observations increase the molecular complexity of RXRs, and indicate that RXR alpha may play a specific function during spermatogenesis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • DNA, Complementary / genetics
  • Gene Expression
  • Male
  • Mice
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • RNA Probes
  • RNA, Antisense
  • RNA, Messenger / analysis
  • RNA, Messenger / genetics
  • Receptors, Retinoic Acid / analysis*
  • Receptors, Retinoic Acid / genetics*
  • Retinoid X Receptors
  • Testis / chemistry*
  • Tissue Distribution
  • Transcription Factors / analysis*
  • Transcription Factors / genetics*
  • Transcription, Genetic


  • DNA, Complementary
  • RNA Probes
  • RNA, Antisense
  • RNA, Messenger
  • Receptors, Retinoic Acid
  • Retinoid X Receptors
  • Transcription Factors

Associated data

  • GENBANK/M84817