Abstract
The induction of CYP1A1 is mediated via the aromatic hydrocarbon (Ah) receptor. Studies from our laboratory show CYP1A1 induction by picrotoxin and phenobarbital which prompted us to examine if other ligands of the gamma-aminobutyric acid (GABA) receptor could also induce CYP1A1. Here we report the nuclear translocation of the Ah receptor and its DNA binding activity to radiolabeled double-stranded synthetic xenobiotic response elements (XREs) in nuclear extracts, increased accumulation of CYP1A1 mRNA, and alterations in intracellular calcium concentrations in cells exposed to GABA receptor ligands.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acids, Diamino / pharmacology
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Animals
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Biological Transport
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Calcium / metabolism
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Cell Compartmentation
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Cells, Cultured
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Cytochrome P-450 CYP1A1 / biosynthesis*
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Cytochrome P-450 CYP1A1 / genetics
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Enzyme Induction
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GABA Antagonists / pharmacology
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Ligands
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Liver / cytology
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Liver / enzymology*
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Oncorhynchus mykiss*
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Phenobarbital / pharmacology
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Polychlorinated Dibenzodioxins / pharmacology
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Protein Binding
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RNA, Messenger / analysis
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Receptors, Aryl Hydrocarbon / metabolism
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Receptors, GABA / metabolism*
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Regulatory Sequences, Nucleic Acid
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gamma-Aminobutyric Acid / pharmacology
Substances
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Amino Acids, Diamino
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GABA Antagonists
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Ligands
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Polychlorinated Dibenzodioxins
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RNA, Messenger
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Receptors, Aryl Hydrocarbon
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Receptors, GABA
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gamma-Aminobutyric Acid
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4,5-diaminopentanoic acid
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Cytochrome P-450 CYP1A1
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Calcium
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Phenobarbital