Smooth muscle alpha-actin expression in rat hepatic stellate cell is regulated by nitric oxide and cGMP production

Biochem Biophys Res Commun. 1996 Dec 4;229(1):238-42. doi: 10.1006/bbrc.1996.1786.


Mechanism by which lipopolysaccharide (LPS) and interferon gamma (IFN gamma) downregulate smooth muscle (SM) alpha-actin expression in rat hepatic stellate cell was studied. Culture-induced SM alpha-actin expression was suppressed in the presence of LPS (> 5 ng/ml) and/or IFN gamma (> 1 U/ml) dose-dependently. Inhibition of nitric oxide (NO) production by N omega-nitro-L-arginine (LNA) canceled such an inhibitory effect of LPS and/or IFN gamma. LPS and/or IFN gamma increased cellular level of cGMP in an LNA inhibitable manner, and cGMP analogues attenuated SM alpha-actin expression of the cells. These results indicate that NO-dependent increase of cellular cGMP level mediates the inhibitory effect of LPS and IFN gamma on SM alpha-actin expression in stellate cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / biosynthesis*
  • Animals
  • Cells, Cultured
  • Cyclic GMP / metabolism*
  • Dose-Response Relationship, Drug
  • Drug Interactions
  • Enzyme Inhibitors / pharmacology
  • Gene Expression
  • Interferon-gamma / pharmacology
  • Lipopolysaccharides / pharmacology
  • Liver / cytology*
  • Liver / drug effects
  • Liver / metabolism*
  • Male
  • Nitric Oxide / metabolism*
  • Nitric Oxide Synthase / antagonists & inhibitors
  • Nitroarginine / pharmacology
  • Rats
  • Rats, Wistar


  • Actins
  • Enzyme Inhibitors
  • Lipopolysaccharides
  • Nitroarginine
  • Nitric Oxide
  • Interferon-gamma
  • Nitric Oxide Synthase
  • Cyclic GMP