Interaction of both the C2A and C2B domains of rabphilin3 with Ca2+ and phospholipid

Biochem Biophys Res Commun. 1996 Dec 13;229(2):498-503. doi: 10.1006/bbrc.1996.1833.


Rabphilin3, a downstream target molecule of the Rab3 subfamily small G proteins, has two C2 domains (the C2A and C2B domains) at its C-terminal region and is implicated in Ca(2+)-dependent neurotransmitter release. Rabphilin3 interacts with Ca2+ and phospholipid at its C2 domain, but it remains to be clarified which domain, the C2A or C2B domain, interacts with these compounds. We have found here that both the C2A and C2B domains interact with Ca2+ with similar kinetics but interact with phospholipid with slightly different kinetics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Animals
  • Calcium / metabolism*
  • Cattle
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / metabolism*
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / metabolism*
  • Phospholipids / metabolism*
  • Protein Binding
  • Vesicular Transport Proteins
  • rab GTP-Binding Proteins*


  • Adaptor Proteins, Signal Transducing
  • Nerve Tissue Proteins
  • Phospholipids
  • Vesicular Transport Proteins
  • rabphilin-3A
  • GTP-Binding Proteins
  • rab GTP-Binding Proteins
  • Calcium