Isolation of four novel neuropeptides, the hydra-RFamides I-IV, from Hydra magnipapillata

Biochem Biophys Res Commun. 1996 Dec 13;229(2):596-602. doi: 10.1006/bbrc.1996.1849.


Using a radioimmunoassay for the sequence Arg-Phe-NH2 (RFamide), we have isolated four novel peptides from extracts of Hydra magnipapillata: <Glu-Trp-Leu-Gly-Gly-Arg-Phe-NH2 (Hydra-RFamide I), <Glu-Trp-Phe-Asn-Gly-Arg-Phe-NH2 (Hydra-RFamide II), Lys-Pro-His-Leu-Arg-Gly-Arg-Phe-NH2 (Hydra-RFamide III) and His-Leu-Arg-Gly-Arg-Phe-NH2 (Hydra-RFamide IV). The Hydra-RFamides I-IV are neuropeptides. They form a peptide family with other known neuropeptides from hydromedusae, sea anemones and sea pansies.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Chromatography, Liquid
  • Chromatography, Thin Layer
  • Hydra / chemistry*
  • Hydrolysis
  • Mass Spectrometry / methods
  • Neuropeptides / chemistry
  • Neuropeptides / isolation & purification*
  • Protein Conformation


  • Neuropeptides