The Intracellular Cytoplasmic Domain of the Alzheimer's Disease Amyloid Precursor Protein Interacts With Phosphotyrosine-Binding Domain Proteins in the Yeast Two-Hybrid System

FEBS Lett. 1996 Nov 18;397(2-3):197-200. doi: 10.1016/s0014-5793(96)01128-3.


We have used the yeast two-hybrid system to screen for proteins that interact with the carboxy-terminal domain of APP. Six different clones were isolated and sequence analyses revealed that they encoded domains of a previously described neuronal protein Fe65, a homologue of Fe65 and a homologue of protein X11. All of these proteins contain one or more phosphotyrosine binding (PTB) domains. PTB domain proteins bind to the sequence Asn-Pro-X-Tyr when the Tyr is phosphorylated and are believed to function in signal transduction. APP contains such a motif. These results are consistent with a role for APP in signal transduction mechanisms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing
  • Amino Acid Sequence
  • Amyloid beta-Protein Precursor / chemistry
  • Amyloid beta-Protein Precursor / metabolism*
  • Animals
  • Cloning, Molecular
  • Cytoplasm / chemistry
  • Humans
  • Membrane Proteins
  • Mice
  • Molecular Sequence Data
  • Nerve Tissue Proteins / chemistry
  • Nerve Tissue Proteins / metabolism*
  • Nuclear Proteins / chemistry
  • Nuclear Proteins / metabolism*
  • Peptide Library
  • Phosphotyrosine / metabolism*
  • Protein Binding
  • Rats
  • Saccharomyces cerevisiae


  • APBA1 protein, human
  • APBB1 protein, human
  • Adaptor Proteins, Signal Transducing
  • Amyloid beta-Protein Precursor
  • Apba1 protein, mouse
  • Apba1 protein, rat
  • Apbb1 protein, mouse
  • Apbb1 protein, rat
  • Membrane Proteins
  • Nerve Tissue Proteins
  • Nuclear Proteins
  • Peptide Library
  • Phosphotyrosine

Associated data

  • GENBANK/L04953
  • GENBANK/L34676
  • GENBANK/X04689