Tissue-specific expression of splice variants of the mouse voltage-gated calcium channel alpha2/delta subunit

FEBS Lett. 1996 Nov 18;397(2-3):331-7. doi: 10.1016/s0014-5793(96)01205-7.


Five different splice variants of mouse alpha2/delta subunit isoforms (alpha2a-e), which arose from various combinations of three alternatively spliced regions, were cloned with a combination of cDNA library screening and RT-PCR. Expression patterns and relative abundance of the various isoforms in mouse tissues were determined with an RNAse protection assay. Skeletal muscle and brain expressed single isoforms, alpha2a and alpha2b, respectively; however, the cardiovascular system expressed all five isoforms. Heart expressed mainly isoforms alpha2c and alpha2d while, in contrast to other species, aorta expressed predominantly alpha2a, the 'skeletal muscle' isoform. Smooth muscle-containing tissues expressed alpha2d and alpha2e. Thus, alpha2/delta isoforms are restricted in their tissue expression, suggesting an important functional role for the differentially spliced variants.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alternative Splicing*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / metabolism
  • Calcium Channels / chemistry
  • Calcium Channels / genetics*
  • Cardiovascular System / metabolism
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Gene Expression*
  • Ion Channel Gating
  • Mice
  • Molecular Sequence Data
  • Muscle, Skeletal / metabolism
  • Muscle, Smooth / metabolism
  • Organ Specificity
  • Polymerase Chain Reaction
  • Sequence Alignment


  • Calcium Channels
  • DNA, Complementary

Associated data

  • GENBANK/L12887
  • GENBANK/M21948
  • GENBANK/M86621
  • GENBANK/U73483
  • GENBANK/U73484
  • GENBANK/U73485
  • GENBANK/U73486
  • GENBANK/U73487