Translation attenuation regulation of chloramphenicol resistance in bacteria--a review

Gene. 1996 Nov 7;179(1):157-62. doi: 10.1016/s0378-1119(96)00420-9.


The chloramphenicol (Cm)-inducible cat and cmlA genes are regulated by translation attenuation, a regulatory device that modulates mRNA translation. In this form of gene regulation, translation of the CmR coding sequence is prevented by mRNA secondary structure that sequesters its ribosome-binding site (RBS). A translated leader of nine codons precedes the secondary structure, and induction results when a ribosome becomes stalled at a specific site in the leader. Here we demonstrate that the site of ribosome stalling in the leader is selected by a cis effect of the nascent leader peptide on its translating ribosome.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Chloramphenicol O-Acetyltransferase / biosynthesis
  • Chloramphenicol O-Acetyltransferase / genetics*
  • Chloramphenicol Resistance / genetics*
  • Gene Expression Regulation, Bacterial*
  • Gram-Negative Bacteria / genetics
  • Gram-Positive Bacteria / genetics
  • Models, Genetic
  • Peptidyl Transferases / antagonists & inhibitors
  • Protein Biosynthesis*
  • RNA, Ribosomal, 23S / metabolism


  • RNA, Ribosomal, 23S
  • Chloramphenicol O-Acetyltransferase
  • Peptidyl Transferases