Purification of protein C a natural anticoagulant, from human plasma by affinity chromatography with Convanavalin A

Prep Biochem Biotechnol. 1996 Aug-Nov;26(3-4):183-8. doi: 10.1080/10826069608000064.

Abstract

We have developed a rapid, efficient, and inexpensive method to purify protein C from plasma using Concanavalin A conjugated to Sepharose. With this method we have obtained protein C purified to homogeneity, as measured in polyacrylamide gels. We discuss each of the steps of our method and give the results of the purification.

MeSH terms

  • Anticoagulants / isolation & purification*
  • Chromatography, Affinity / methods
  • Concanavalin A
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Protein C / isolation & purification*

Substances

  • Anticoagulants
  • Protein C
  • Concanavalin A