In-vivo clearance study of vancomycin in rats

J Pharm Pharmacol. 1996 Nov;48(11):1197-200. doi: 10.1111/j.2042-7158.1996.tb03920.x.


The renal handling of vancomycin in rats and the effects of various drugs (probenecid, cimetidine and quinidine) on urinary excretion of the antibiotic were investigated by in-vivo clearance. The vancomycin-to-inulin excretion ratio (ER) was greater than unity at various infusion rates of vancomycin. Quinidine, co-administered with vancomycin, significantly decreased the total, renal and net secretory clearance of the antibiotic. Cimetidine also decreased, though not significantly, the secretory clearance of vancomycin by about 20%, but probenecid did not. These results suggested that vancomycin is secreted in renal tubules in rats, and that quinidine decreases the total clearance of vancomycin partly by inhibiting its tubular secretion in the kidney.

MeSH terms

  • Analysis of Variance
  • Animals
  • Anti-Bacterial Agents / blood
  • Anti-Bacterial Agents / pharmacokinetics*
  • Anti-Bacterial Agents / urine
  • Cimetidine / pharmacology
  • Kidney / drug effects
  • Kidney / metabolism*
  • Male
  • Metabolic Clearance Rate
  • Probenecid / pharmacology
  • Quinidine / pharmacology
  • Rats
  • Rats, Wistar
  • Renal Agents / pharmacology
  • Vancomycin / blood
  • Vancomycin / pharmacokinetics*
  • Vancomycin / urine


  • Anti-Bacterial Agents
  • Renal Agents
  • Vancomycin
  • Cimetidine
  • Quinidine
  • Probenecid