Mechanism of action of hemolysin III from Bacillus cereus

Biochim Biophys Acta. 1996 Oct 23;1284(2):122-4. doi: 10.1016/s0005-2736(96)00168-x.

Abstract

Bacillus cereus hemolysin III activity was tested in crude extracts, from Escherichia coli carrying the hly-III gene. It was concluded that hemolysin III is a pore-forming hemolysin with functional pore diameter of about 3-3.5 nm. Hemolysis occurs in at least three steps: (i) the temperature-dependent binding of the Hly-III monomers to the erythrocyte membrane; (ii) the temperature-dependent formation of the transmembrane oligomeric pore; (iii) the temperature-independent erythrocyte lysis.

MeSH terms

  • Bacillus cereus
  • Cloning, Molecular
  • Erythrocyte Membrane / physiology*
  • Erythrocytes / drug effects
  • Erythrocytes / physiology
  • Escherichia coli
  • Genes, Bacterial
  • Hemolysin Proteins / isolation & purification
  • Hemolysin Proteins / pharmacology*
  • Hemolysis*
  • Humans
  • Osmolar Concentration
  • Protein Binding
  • Recombinant Proteins / isolation & purification
  • Recombinant Proteins / pharmacology
  • Temperature

Substances

  • Hemolysin Proteins
  • Recombinant Proteins