In vitro swelling studies and preliminary biocompatibility evaluation of acrylamide-based hydrogels

Biomaterials. 1996 Jan;17(1):67-70. doi: 10.1016/0142-9612(96)80757-5.


In this in vitro study, the biocompatibility of acrylamide-based hydrogels such as acrylamide/crotonic and acrylamide/itaconic acid, prepared by using gamma-rays, has been investigated against some biochemical parameters of human serum. The swelling kinetics of acrylamide/crotonic and acrylamide/itaconic acid hydrogels are investigated in distilled water and physiological saline solution and some swelling and diffusion parameters have been calculated. For the analysis of biocompatibility, acrylamide/crotonic and acrylamide/itaconic acid hydrogels are incubated in 10 different human sera for 24 h and their biocompatibilities with some biochemical parameters have been investigated. No significant difference in values before and after the test procedures have been found. It is therefore concluded that acrylamide/crotonic and acrylamide/itaconic acid hydrogels are biocompatible.

MeSH terms

  • Acrylamide
  • Acrylamides / chemistry
  • Acrylamides / metabolism*
  • Biocompatible Materials / standards
  • Blood Proteins / metabolism*
  • Crotonates / chemistry*
  • Crotonates / metabolism
  • Diffusion
  • Gels
  • Humans
  • In Vitro Techniques
  • Kinetics
  • Protein Binding
  • Succinates / chemistry*
  • Succinates / metabolism


  • Acrylamides
  • Biocompatible Materials
  • Blood Proteins
  • Crotonates
  • Gels
  • Succinates
  • Acrylamide
  • itaconic acid