Heat-stable serotyping antigens expressed by strains of Campylobacter jejuni are probably capsular and not long-chain lipopolysaccharide

J Appl Bacteriol. 1996 Dec;81(6):635-40. doi: 10.1111/j.1365-2672.1996.tb03558.x.

Abstract

The role of lipopolysaccharide (LPS) in the serotyping of Campylobacter jejuni based on heat-stable antigens was examined using SDS-PAGE and a silver stain for carbohydrate. None of the 32 type strains of Camp. jejuni expressed long-chain LPS. Rabbit antibodies, prepared to 10 selected strains of Camp. jejuni, reacted with surface-exposed carbohydrate antigens, which were not LPS. This study suggests that the heat-stable antigens of Camp. jejuni, which form the basis for the established Penner serotyping scheme, are probably capsular and not LPS.

MeSH terms

  • Animals
  • Antibody Specificity
  • Antigens, Bacterial / analysis*
  • Bacterial Capsules / chemistry*
  • Bacterial Capsules / immunology
  • Campylobacter jejuni / chemistry*
  • Campylobacter jejuni / classification*
  • Campylobacter jejuni / immunology
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Escherichia coli O157 / immunology
  • Hemagglutinins
  • Hot Temperature
  • Immunoblotting
  • Lipopolysaccharides / analysis
  • Lipopolysaccharides / immunology
  • Rabbits
  • Serotyping

Substances

  • Antigens, Bacterial
  • Hemagglutinins
  • Lipopolysaccharides