Abstract
The cellular interleukin-6 (IL-6) gene contains a target site for the mammalian transcriptional repressor RBP. The target site is contained within the interleukin response element (ILRE), which mediates IL-6 activation by NF-kappa B. In this study, we show by using transient-expression assays that RBP represses activated transcription from the IL-6 gene. The presence and position of the RBP target site are crucial in mediating repression by RBP. While RBP binds within the ILRE, it does not target NF-kappa B alone; nonetheless, NF-kappa B binding to the ILRE is required for repression. Our results indicate that RBP represses coactivation by NF-kappa B and another cellular transcription factor, C/EBP-beta.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Binding Sites
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CCAAT-Enhancer-Binding Proteins
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COS Cells
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Cell Line
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / metabolism*
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DNA-Binding Proteins / pharmacology
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Gene Expression Regulation / physiology*
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Immunoglobulin J Recombination Signal Sequence-Binding Protein
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Interleukin-6 / genetics*
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Interleukins
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Mice
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Mutation
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NF-kappa B / pharmacology
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Nuclear Proteins / pharmacology
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Promoter Regions, Genetic / genetics
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RNA, Messenger / biosynthesis
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Repressor Proteins / genetics
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Repressor Proteins / metabolism*
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Transcription Factor RelA
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Transcription, Genetic / genetics
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Transcriptional Activation / drug effects
Substances
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CCAAT-Enhancer-Binding Proteins
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DNA-Binding Proteins
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Immunoglobulin J Recombination Signal Sequence-Binding Protein
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Interleukin-6
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Interleukins
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NF-kappa B
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Nuclear Proteins
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RNA, Messenger
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Rbpj protein, mouse
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Repressor Proteins
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Transcription Factor RelA