Analysis of a 23 kb region on the left arm of yeast chromosome IV

Yeast. 1996 Dec;12(15):1587-92. doi: 10.1002/(SICI)1097-0061(199612)12:15%3C1587::AID-YEA46%3E3.0.CO;2-6.

Abstract

We have determined the complete nucleotide sequence of a 23 kb segment from the left arm of chromosome IV, which is carried by the cosmid 1L10. This sequence contains the 3' coding region of the STE7 and RET1 (COP1) genes, and 13 complete open reading frames longer than 300 bp, of which ten correspond to putative new genes and three (CLB3, MSH5 and RPC53) have been sequenced previously. The sequence from cosmid IL10 was obtained entirely by a combined subcloning and walking primer strategy.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Chromosome Mapping
  • Chromosomes / genetics*
  • Cloning, Molecular
  • Coatomer Protein
  • Cosmids / genetics
  • Cyclins / genetics
  • DNA, Fungal / analysis*
  • Electronic Data Processing
  • Fungal Proteins / genetics
  • Gene Library
  • Membrane Proteins / genetics
  • Molecular Sequence Data
  • Open Reading Frames
  • RNA Polymerase III / genetics
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae Proteins*
  • Sequence Analysis, DNA*
  • Transcription Factors / genetics
  • Yeasts / genetics*

Substances

  • Coatomer Protein
  • Cyclins
  • DNA, Fungal
  • Fungal Proteins
  • MSH5 protein, S cerevisiae
  • Membrane Proteins
  • STE12 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Transcription Factors
  • RNA Polymerase III

Associated data

  • GENBANK/X97751