Genomic organization and promoter analysis of the gene ifngr2 encoding the second chain of the mouse interferon-gamma receptor

Scand J Immunol. 1996 Dec;44(6):599-606. doi: 10.1046/j.1365-3083.1996.d01-353.x.

Abstract

A clone containing the gene ifngr2 for the second chain (IFN-gamma R2) of the mouse interferon gamma receptor complex was isolated from a cosmid library made of 129/Sv mouse genomic DNA. Sequence analysis revealed that the second chain is encoded by 7 exons. The complete gene spans about 17 kb of the genomic DNA. In the 5'-flanking region several transcription initiation sites between 27 and 136 nucleotides upstream from the translation initiation codon were mapped. This region has a high GC content, but no TATA or CAAT box. Potential binding sites were found for transcription factors Sp1, AP-2, NF1, EGR and NF kappa B. Promoter activity was assayed with a series of constructs with firefly luciferase as a reporter gene, under the control of the promoter fragments of various lengths. This region showed promoter activity in transiently transfected Chinese hamster ovary cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, CD / genetics*
  • Base Sequence
  • Binding Sites
  • CHO Cells
  • Cricetinae
  • DNA / metabolism
  • Exons
  • Genome
  • Mice
  • Molecular Sequence Data
  • Promoter Regions, Genetic*
  • Receptors, Interferon / genetics*
  • Transcription Factors / metabolism

Substances

  • Antigens, CD
  • Receptors, Interferon
  • Transcription Factors
  • interferon gamma receptor
  • DNA

Associated data

  • GENBANK/U69594
  • GENBANK/U69595
  • GENBANK/U69596
  • GENBANK/U69597
  • GENBANK/U69598
  • GENBANK/U69599