Novel Phosphotransferase-Encoding Genes Revealed by Analysis of the Escherichia Coli Genome: A Chimeric Gene Encoding an Enzyme I Homologue That Possesses a Putative Sensory Transduction Domain

Gene. 1996 Nov 28;181(1-2):103-8. doi: 10.1016/s0378-1119(96)00481-7.

Abstract

Two genes (ptsI and ptsA) that encode homologues of the energy coupling Enzyme I of the phosphoenolpyruvate-dependent sugar-transporting phosphotransferase system (PTS) have previously been identified on the Escherichia coli chromosome. We here report the presence of a third E. coli gene, designated ptsP, that encodes an Enzyme I homologue, here designated Enzyme INtr. Enzyme INtr possesses an N-terminal domain homologous to the N-terminal domains of NifA proteins [(127 amino acids (aa)] joined via two tandem flexible linkers to the C-terminal Enzyme I-like domain (578 aa). Structural features of the putative ptsP operon, including transcriptional regulatory signals, are characterized. We suggest that Enzyme INtr functions in transcriptional regulation of nitrogen-related operons together with previously described PTS proteins encoded within the rpoN operon. It may thereby provide a link between carbon and nitrogen assimilatory pathways.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Bacterial Proteins / classification
  • Bacterial Proteins / genetics*
  • Bacterial Proteins / metabolism
  • Binding Sites / genetics
  • Cricetinae
  • DNA-Binding Proteins*
  • DNA-Directed RNA Polymerases / genetics
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Escherichia coli Proteins
  • Genome, Bacterial
  • Molecular Sequence Data
  • Multigene Family
  • Operon
  • Phosphoenolpyruvate Sugar Phosphotransferase System / chemistry*
  • Phosphoenolpyruvate Sugar Phosphotransferase System / classification
  • Phosphoenolpyruvate Sugar Phosphotransferase System / genetics*
  • Phosphotransferases (Nitrogenous Group Acceptor) / chemistry*
  • Phosphotransferases (Nitrogenous Group Acceptor) / classification
  • Phosphotransferases (Nitrogenous Group Acceptor) / genetics*
  • Phylogeny
  • RNA Polymerase Sigma 54
  • Recombinant Fusion Proteins / genetics
  • Regulatory Sequences, Nucleic Acid
  • Salmonella typhimurium / enzymology
  • Sequence Homology, Amino Acid
  • Sigma Factor / genetics
  • Transcription Factors / metabolism
  • Transcription, Genetic

Substances

  • Bacterial Proteins
  • DNA-Binding Proteins
  • Escherichia coli Proteins
  • NifA protein, Bacteria
  • Recombinant Fusion Proteins
  • Sigma Factor
  • Transcription Factors
  • rpoN protein, E coli
  • Phosphoenolpyruvate Sugar Phosphotransferase System
  • Phosphotransferases (Nitrogenous Group Acceptor)
  • phosphoenolpyruvate-protein phosphotransferase
  • DNA-Directed RNA Polymerases
  • RNA Polymerase Sigma 54

Associated data

  • GENBANK/D13799
  • GENBANK/L15191
  • GENBANK/P03027
  • GENBANK/P08838
  • GENBANK/P08839
  • GENBANK/P11155
  • GENBANK/P12626
  • GENBANK/P12627
  • GENBANK/P22221
  • GENBANK/P22983
  • GENBANK/P23388
  • GENBANK/P23533
  • GENBANK/P23536
  • GENBANK/P23538
  • GENBANK/P27713
  • GENBANK/P30299
  • GENBANK/P30667
  • GENBANK/P37213
  • GENBANK/P42014
  • GENBANK/P42850
  • GENBANK/P43922
  • GENBANK/S49497
  • GENBANK/S74619
  • GENBANK/U00078
  • GENBANK/U08399
  • GENBANK/U08400
  • GENBANK/U15110
  • GENBANK/U29581
  • GENBANK/X75516
  • GENBANK/Z37113