Cloning of a cDNA encoding the murine orphan receptor RZR/ROR gamma and characterization of its response element

Gene. 1996 Nov 28;181(1-2):199-206. doi: 10.1016/s0378-1119(96)00504-5.


In this study, we describe the cloning of the mouse homologue of the orphan receptor, RZR/ROR gamma, a member of the nuclear receptor superfamily, from a mouse muscle cDNA library. The amino acid sequence of mouse ROR gamma (mROR gamma) is highly homologous to that of human ROR gamma, with an overall identity of 88%. Northern blot analysis using RNA from different tissues showed that mROR gamma was found to be highly expressed in skeletal muscle, liver and kidney. Analysis of the ROR gamma-response element using in vitro synthesized ROR gamma revealed that it binds as a monomer to response elements composed of a single core motif GGTCA preceded by a 6 bp AT-rich sequence. The ROR gamma-binding specificity was further defined by mutational analysis of the consensus RORE. ROR gamma was able to activate RORE-dependent transcription of the CAT reporter gene in mouse fibroblast D1 cells. ROR alpha 1 and ROR gamma inhibit the transactivation induced by GAL4(DBD)-ROR gamma in fibroblast D1 cells suggesting that these receptors compete for binding to the same coactivators.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • Chloramphenicol O-Acetyltransferase / genetics
  • Cloning, Molecular
  • Consensus Sequence
  • DNA, Complementary
  • Genes, Reporter
  • Humans
  • Mice
  • Molecular Sequence Data
  • Mutagenesis
  • Nuclear Receptor Subfamily 1, Group F, Member 3
  • Receptors, Cytoplasmic and Nuclear / genetics*
  • Receptors, Cytoplasmic and Nuclear / metabolism
  • Receptors, Retinoic Acid*
  • Receptors, Thyroid Hormone*
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Transcriptional Activation


  • DNA, Complementary
  • Nuclear Receptor Subfamily 1, Group F, Member 3
  • RORC protein, human
  • Receptors, Cytoplasmic and Nuclear
  • Receptors, Retinoic Acid
  • Receptors, Thyroid Hormone
  • Rorc protein, mouse
  • Chloramphenicol O-Acetyltransferase

Associated data

  • GENBANK/AF019655
  • GENBANK/AF019656
  • GENBANK/AF019657
  • GENBANK/AF019658
  • GENBANK/AF019659
  • GENBANK/AF019660
  • GENBANK/U43508