Unusual strain of Borrelia burgdorferi isolated from Ixodes dentatus in central Georgia

J Parasitol. 1996 Dec;82(6):936-40.

Abstract

A new, unusual spirochete was cultured in Barbour-Stoenner-Kelly (BSK II) medium from the midgut and other tissues of the tick Ixodes dentatus. The tick was collected from leaf litter in an oak-pine wood lot in Bibb County approximately 7.2 km from Macon in central Georgia during February 1993. Characterization by indirect immunofluorescence using 5 murine monoclonal antibodies, by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole spirochetal lysates, and by polymerase chain reaction assay for several known DNA target sequences indicates that the spirochete is Borrelia burgdorferi sensu lato. It is genetically different from the B-31 reference strain of B. burgdorferi sensu stricto that is typical of strains causing Lyme borreliosis in North America. Range of infectivity and pathogenesis of the Bibb County isolate (BC-1) are unknown but being investigated. The BC-1 strain is the first B. burgdorferi isolate from I. dentatus in the southeastern United States (I. dentatus is not the common vector for Lyme borreliosis in humans). Additionally, the collection site was approximately 322 km from the Atlantic coast, far distant from where most B. burgdorferi isolates have been obtained.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Antigens, Bacterial*
  • Antigens, Surface / analysis
  • Antigens, Surface / genetics
  • Arachnid Vectors / microbiology*
  • Bacterial Outer Membrane Proteins / analysis
  • Bacterial Outer Membrane Proteins / genetics
  • Bacterial Vaccines
  • Borrelia burgdorferi Group / genetics*
  • Borrelia burgdorferi Group / immunology
  • Borrelia burgdorferi Group / isolation & purification
  • DNA Primers / chemistry
  • DNA, Bacterial / analysis
  • Electrophoresis, Agar Gel
  • Electrophoresis, Polyacrylamide Gel
  • Flagellin / genetics
  • Fluorescent Antibody Technique, Indirect
  • Georgia
  • Ixodes / microbiology*
  • Lipoproteins*
  • Polymerase Chain Reaction

Substances

  • Antigens, Bacterial
  • Antigens, Surface
  • Bacterial Outer Membrane Proteins
  • Bacterial Vaccines
  • DNA Primers
  • DNA, Bacterial
  • Lipoproteins
  • OspA protein
  • Flagellin
  • OspB protein, Borrelia burgdorferi