Effects of cannabinoids on L1210 murine leukemia. 1. Inhibition of DNA synthesis

Res Commun Chem Pathol Pharmacol. 1977 Aug;17(4):703-14.

Abstract

The effect of cannabinoid derivatives on thymidine-3H uptake in L1210 murine leukemia was determined. In experiments at 200 mg/kg 3 hrs after treatment, the order of activity was delta9-tetrahydrocannabinol less than cannabinol less than cannabidiol less than abnormal cannabidiol less than 11-hydroxy-delta9-tetrahydrocannabinol less than delta8-tetrahydrocannabinol. The inhibitory effect of delta8-tetrahydrocannabinol was 99%. When animals were dosed on consecutive days with delta9-tetrahydrocannabinol and killed on the third day, thymidine-3H incorporation was increased while delta8-tetrahydrocannabinol retained its inhibitory activity under the same conditions. Delta-9-tetrahydrocannabinol and delta8-tetrahydrocannabinol inhibited RNA and protein synthesis in a fashion analagous to the inhibition of DNA synthesis.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cannabinoids / pharmacology*
  • DNA, Neoplasm / biosynthesis*
  • Depression, Chemical
  • Dronabinol / pharmacology
  • Leukemia L1210 / metabolism*
  • Mice
  • Mice, Inbred C57BL
  • Mice, Inbred DBA
  • Neoplasm Proteins / biosynthesis
  • RNA, Neoplasm / biosynthesis
  • Thymidine / metabolism
  • Time Factors

Substances

  • Cannabinoids
  • DNA, Neoplasm
  • Neoplasm Proteins
  • RNA, Neoplasm
  • Dronabinol
  • Thymidine