M-current suppression in PC12 cells by bradykinin is mediated by a pertussis toxin-insensitive G-protein and modulated by intracellular calcium

Brain Res. 1996 Nov 18;740(1-2):227-33. doi: 10.1016/s0006-8993(96)00870-0.

Abstract

Inhibition of the M-current in PC12 cells by bradykinin has been studied under whole-cell recording conditions. In cells dialyzed with GTP-gamma-S, bradykinin produced a total and irreversible M-current suppression, while the inhibition was attenuated in cells dialyzed with GDP-beta-S. Inhibition occurred in cells pretreated with pertussis toxin, while this treatment prevented the modulation of Ca2+ currents by muscarine. The rate and extent of inhibition increased with the level of intracellular Ca2+ from 0 to 70 nM. These results indicate that a pertussis toxin insensitive G-protein mediates the action of bradykinin on the M-current, and some steps in the second messenger cascade are modulated by Ca2+.

MeSH terms

  • Animals
  • Bradykinin / pharmacology*
  • Calcium / pharmacology*
  • GTP-Binding Proteins / drug effects*
  • Membrane Potentials / drug effects*
  • Muscarine / pharmacology
  • PC12 Cells / drug effects*
  • Patch-Clamp Techniques
  • Pertussis Toxin*
  • Rats
  • Virulence Factors, Bordetella / pharmacology*

Substances

  • Virulence Factors, Bordetella
  • Muscarine
  • Pertussis Toxin
  • GTP-Binding Proteins
  • Bradykinin
  • Calcium