The human GATA-6 gene: structure, chromosomal location, and regulation of expression by tissue-specific and mitogen-responsive signals

Genomics. 1996 Dec 15;38(3):283-90. doi: 10.1006/geno.1996.0630.


GATA factors constitute a family of transcriptional regulatory proteins expressed with distinct developmental and tissue-specific profiles and thought to regulate cell-restricted programs of gene expression. Here we describe the molecular cloning, chromosomal location, and transcription of the human GATA-6 gene. The GATA-6 cDNA encodes a predicted 449-amino-acid protein, which is highly conserved among vertebrates, and includes the two adjacent zinc-finger/basic domains characteristic of the GATA factor family. GATA-6 maps to human chromosome 18q11.1-q11.2 by fluorescence in situ hybridization. The gene is transcribed in a pattern overlapping that of GATA-4. Transcripts for both of these genes are prominent in heart, pancreas, and ovary, but only GATA-6 mRNA is found in lung and liver. GATA-6 transcripts are also detected in cultures of human and rat vascular smooth muscle cells (VSMCs). In VSMCs, GATA-6 transcripts are down- regulated when quiescent cultures are stimulated to proliferate in response to mitogen activation. These data demonstrate that GATA-6 is subject to both tissue-specific and mitogen-responsive regulatory signals. GATA-6 is a prime candidate for a gene that might regulate the differentiative state of VSMCs.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cattle
  • Cells, Cultured
  • Chromosome Mapping
  • Chromosomes, Human, Pair 18
  • DNA-Binding Proteins / genetics*
  • Fetal Heart / chemistry
  • GATA6 Transcription Factor
  • Gene Expression Regulation / drug effects*
  • Genes*
  • Humans
  • In Situ Hybridization, Fluorescence
  • Male
  • Mitogens / pharmacology*
  • Molecular Sequence Data
  • Muscle Proteins / biosynthesis
  • Muscle Proteins / genetics
  • Muscle, Smooth, Vascular / cytology
  • Muscle, Smooth, Vascular / metabolism
  • Organ Specificity
  • RNA, Messenger / analysis
  • Rats
  • Rats, Sprague-Dawley
  • Transcription Factors / genetics*
  • Trinucleotide Repeats
  • Zinc Fingers / genetics


  • DNA-Binding Proteins
  • GATA6 Transcription Factor
  • GATA6 protein, human
  • Mitogens
  • Muscle Proteins
  • RNA, Messenger
  • Transcription Factors

Associated data

  • GENBANK/U66075