HLA-DM interactions with intermediates in HLA-DR maturation and a role for HLA-DM in stabilizing empty HLA-DR molecules

J Exp Med. 1996 Dec 1;184(6):2153-65. doi: 10.1084/jem.184.6.2153.


Major histocompatibility complex (MHC) class II-positive cell lines which lack HLA-DM expression accumulate class II molecules associated with residual invariant (I) chain fragments (class II-associated invariant chain peptides [CLIP]). In vitro, HLA-DM catalyzes CLIP dissociation from class II-CLIP complexes, promoting binding of antigenic peptides. Here the physical interaction of HLA-DM with HLA-DR molecules was investigated. HLA-DM complexes with class II molecules were detectable transiently in cells, peaking at the time when the class II molecules entered the MHC class II compartment. HLA-DR alpha beta dimers newly released from I chain, and those associated with I chain fragments, were found to associate with HLA-DM in vivo. Mature, peptide-loaded DR molecules also associated at a low level. These same species, but not DR-I chain complexes, were also shown to bind to purified HLA-DM molecules in vitro. HLA-DM interaction was quantitatively superior with DR molecules isolated in association with CLIP. DM-DR complexes generated by incubating HLA-DM with purified DR alpha beta CLIP contained virtually no associated CLIP, suggesting that this superior interaction reflects a prolonged HLA-DM association with empty class II dimers after CLIP dissociation. Incubation of peptide-free alpha beta dimers in the presence of HLA-DM was found to prolong their ability to bind subsequently added antigenic peptides. Stabilization of empty class II molecules may be an important property of HLA-DM in facilitating antigen processing.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Cell Line, Transformed
  • Dimerization
  • HLA-D Antigens / biosynthesis
  • HLA-D Antigens / immunology*
  • HLA-DR Antigens / immunology*
  • HLA-DR3 Antigen / biosynthesis
  • HLA-DR3 Antigen / chemistry
  • HLA-DR3 Antigen / immunology*
  • Histocompatibility Antigens Class II*
  • Humans
  • Kinetics
  • Molecular Sequence Data
  • Peptide Fragments / chemical synthesis
  • Peptide Fragments / immunology
  • Protein Binding
  • Time Factors
  • Transfection
  • Tumor Cells, Cultured


  • H2-M antigens
  • HLA-D Antigens
  • HLA-DM antigens
  • HLA-DR Antigens
  • HLA-DR3 Antigen
  • Histocompatibility Antigens Class II
  • Peptide Fragments