Characterization of a human gene related to genes encoding somatostatin receptors

FEBS Lett. 1996 Dec 2;398(2-3):253-8. doi: 10.1016/s0014-5793(96)01160-x.


We report the identification of a gene, named SLC-1(1), encoding a novel G protein-coupled receptor (GPCR). A customized search procedure of a database of expressed sequence tags (dbEST) retrieved a human cDNA sequence that partially encoded a GPCR. A genomic DNA fragment identical to the cDNA was obtained and used to screen a library to isolate the full-length coding region of the gene. This gene was intronless in its open reading frame, and encoded a receptor of 402 amino acids, and shared -40% amino acid identity in the transmembrane (TM) regions to the five known human somatostatin receptors. Northern blot analysis revealed that SLC-1 is expressed in human brain regions, including the forebrain and hypothalamus. Expression in the rat was highest in brain, followed by heart, kidney, and ovary. Expression of SLC-1 in COS-7 cells failed to show specific binding to radiolabelled Tyr1-somatostatin-14, naloxone, bremazocine, 1,3-di(2-tolyl)-guanidine (DTG), or haloperidol. A repeat polymorphism of the form (CA)n was discovered in the 5'-untranslated region (UTR) of the gene and SLC-1 was mapped to chromosome 22, q13.3.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Blotting, Northern
  • Brain / metabolism
  • COS Cells
  • Chromosome Mapping
  • Chromosomes, Human, Pair 22
  • Cloning, Molecular
  • DNA, Complementary / genetics
  • Dinucleotide Repeats
  • GTP-Binding Proteins / chemistry
  • GTP-Binding Proteins / genetics*
  • GTP-Binding Proteins / metabolism
  • Gene Expression
  • Genomic Library
  • Humans
  • In Situ Hybridization, Fluorescence
  • Introns
  • Ligands
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Rats
  • Receptors, Somatostatin / chemistry
  • Receptors, Somatostatin / genetics*
  • Receptors, Somatostatin / metabolism
  • Somatostatin / analogs & derivatives
  • Somatostatin / metabolism


  • DNA, Complementary
  • Ligands
  • MCHR1 protein, human
  • MCHR1 protein, rat
  • Receptors, Somatostatin
  • Somatostatin
  • somatostatin, N-Tyr(1)-
  • GTP-Binding Proteins

Associated data

  • GENBANK/U71092
  • GENBANK/U77953