[Methods for the determination of the sensitivity of Candida strains to fluconazole: focus of a micromethod and correlation with 2 other techniques]

Pathol Biol (Paris). 1996 Oct;44(8):724-8.
[Article in French]

Abstract

107 Strains of different species of Candida have been tested by three methods of susceptibility to fluconazole. In addition, one reference strain has been used to verify three methods' reliability: one diffusion method on Shadomy's medium and two MIC liquid methods, on tube and on microtiter plate. Method on Shadomy's medium seems less sensitive for yeast susceptibility. Comparison of two liquid method of MIC show discordances on resistant strains (14% for method on tube and 26% for micromethod) By simplicity of execution and reliability in detection of natural resistances, micromethod of MIC seems the most convenient for yeasts determination in vitro to fluconazole.

Publication types

  • Comparative Study
  • English Abstract

MeSH terms

  • Antifungal Agents / pharmacology*
  • Candida / classification
  • Candida / drug effects*
  • Fluconazole / pharmacology*
  • Immunodiffusion
  • In Vitro Techniques
  • Microbial Sensitivity Tests / methods*

Substances

  • Antifungal Agents
  • Fluconazole